Comprehensive Review of Transcriptomics (RNAs) Workflows from Blood Specimens

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dc.authoridTATLISU, Nevruz Berna/0000-0001-7460-1560
dc.authoridHusseini, Abbas Ali/0000-0002-8861-7106
dc.contributor.authorHusseini, Abbas Ali
dc.contributor.authorDerakhshandeh, Masoud
dc.contributor.authorTatlisu, Nevruz Berna
dc.date.accessioned2024-09-11T19:51:35Z
dc.date.available2024-09-11T19:51:35Z
dc.date.issued2022
dc.departmentİstanbul Gelişim Üniversitesien_US
dc.description.abstractBlood is the most interesting tissue for molecular analysis in a wide range of diseases since it provides accumulative information of the whole body state. Transcriptomic analysis of the blood is one of the most favorable omics approaches for clinical and research purposes. However, blood sample preparation for RNA analyses is highly diverse encompassing specific methodology and application. In this study, we review the common sample preparation maneuvers including RNA handling techniques, sample collection and stabilization, blood cell sorting, extracellular vesicle enrichment, RNA extraction, globin/ribosomal RNA reduction, and RNA concentration and quality assessment. These techniques are the most prevalent procedures of blood sample preparation in RNA transcriptomic analyses. The methodologies, technical challenges of the current approaches, and latest advances in the field are discussed in detail.en_US
dc.identifier.doi10.1080/15422119.2020.1831537
dc.identifier.endpage77en_US
dc.identifier.issn1542-2119
dc.identifier.issn1542-2127
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-85098670270en_US
dc.identifier.startpage57en_US
dc.identifier.urihttps://doi.org/10.1080/15422119.2020.1831537
dc.identifier.urihttps://hdl.handle.net/11363/7819
dc.identifier.volume51en_US
dc.identifier.wosWOS:000604006700001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofSeparation And Purification Reviewsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz20240903_Gen_US
dc.subjectTranscriptomic analysisen_US
dc.subjectRNA handling methodsen_US
dc.subjectblooden_US
dc.subjectworkflowen_US
dc.titleComprehensive Review of Transcriptomics (RNAs) Workflows from Blood Specimensen_US
dc.typeReview Articleen_US

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