Developing a surface acoustic wave-induced microfluidic cell lysis device for point-of-care DNA amplification

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dc.authoridHusseini, Abbas Ali/0000-0002-8861-7106
dc.contributor.authorHusseini, Abbas Ali
dc.contributor.authorYazdani, Ali Mohammad
dc.contributor.authorGhadiri, Fatemeh
dc.contributor.authorSisman, Alper
dc.date.accessioned2024-09-11T19:50:05Z
dc.date.available2024-09-11T19:50:05Z
dc.date.issued2024
dc.departmentİstanbul Gelişim Üniversitesien_US
dc.description.abstractWe developed a microchip device using surface acoustic waves (SAW) and sharp-edge glass microparticles to rapidly lyse low-level cell samples. This microchip features a 13-finger pair interdigital transducer (IDT) with a 30-degree focused angle, creating high-intensity acoustic beams converging 6 mm away at a 16 MHz frequency. Cell lysis is achieved through centrifugal forces acting on Candida albicans cells and glass particles within the focal area. To optimize this SAW-induced streaming, we conducted 42 pilot experiments, varying electrical power, droplet volume, glass particle size, concentration, and lysis time, resulting in optimal conditions: an electrical signal of 2.5 W, a 20 mu L sample volume, glass particle size below 10 mu m, concentration of 0.2 mu g, and a 5-min lysis period. We successfully amplified DNA target fragments directly from the lysate, demonstrating an efficient microchip-based cell lysis method. When combined with an isothermal amplification technique, this technology holds promise for rapid point-of-care (POC) applications.en_US
dc.description.sponsorshipTUBITAK [121M969]; Istanbul Gelisim University Scientific Research Projects Application and Research Center [KAP-050421-AHH]en_US
dc.description.sponsorshipTUBITAK, Grant/Award Number: 121M969; Istanbul Gelisim University Scientific Research Projects Application and Research Center, Grant/Award Number: KAP-050421-AHHen_US
dc.identifier.doi10.1002/elsc.202300230
dc.identifier.issn1618-0240
dc.identifier.issn1618-2863
dc.identifier.issue1en_US
dc.identifier.pmid38187928en_US
dc.identifier.scopus2-s2.0-85178412744en_US
dc.identifier.urihttps://doi.org/10.1002/elsc.202300230
dc.identifier.urihttps://hdl.handle.net/11363/7555
dc.identifier.volume24en_US
dc.identifier.wosWOS:001113996600001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofEngineering In Life Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240903_Gen_US
dc.subjectcell lysisen_US
dc.subjectdropleten_US
dc.subjectmicro-glass particleen_US
dc.subjectsurface acoustic waveen_US
dc.titleDeveloping a surface acoustic wave-induced microfluidic cell lysis device for point-of-care DNA amplificationen_US
dc.typeArticleen_US

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