Gundogan, Gul IpekIrez, TulayBozkurt, Hasan Hakan2024-09-112024-09-1120221698-031Xhttps://doi.org/10.1016/j.androl.2021.04.003https://hdl.handle.net/11363/8381Introduction: Fertilin ? is a sperm surface protein that can mediate sperm-egg membrane interaction. This study was conducted to determine whether the expression of fertilin ? after intrauterine insemination (IUI) in donors with normal parameters after standard semen analysis is related to low success rate or failure of fertilization. Methods: We examined the sperm of 30 male donors who have normal as controls, oligozoospermia, and unexplained infertility as the clinically indication for IUI. Fertilin ? has been labeled with the ADAM2 antibody by indirect immunofluorescence (IF) assay. To evaluate the reproducibility of the test, we selected four sperm samples scale of 0 to +++ according to the distribution of fluorescence label. Results: The results were highly correlated with the corrected total cell fluorescence (CTCF) (Rp = 0.9972, P < 0.05). We suggest that the relationship between infertility and fertilin ? may be due to the distribution of this protein on the sperm surface. Male partners of couples with unexplained infertility showed a low distribution of fertilin ? by a decrease of the fluorescence signal in the IF labeling (scale of +++ by 7.4 ± 10.32%, P < 0.0001, ±SD). Discussion: Abnormal fertilin ? function may be a potential mechanism that could lead to fertilization failure. © 2022 Asociación Española de Andrología, Medicina Sexual y Reproductivaeninfo:eu-repo/semantics/closedAccessAndrology; Fertility; Oligozoospermia; Sperm; Surface proteins; Unexplained infertilityArticle20424024810.1016/j.androl.2021.04.003359332932-s2.0-85135388951Q3