Is there a relationship between infertility and fertilin ? protein distribution?
Küçük Resim Yok
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Ediciones Doyma, S.L.
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Introduction: Fertilin ? is a sperm surface protein that can mediate sperm-egg membrane interaction. This study was conducted to determine whether the expression of fertilin ? after intrauterine insemination (IUI) in donors with normal parameters after standard semen analysis is related to low success rate or failure of fertilization. Methods: We examined the sperm of 30 male donors who have normal as controls, oligozoospermia, and unexplained infertility as the clinically indication for IUI. Fertilin ? has been labeled with the ADAM2 antibody by indirect immunofluorescence (IF) assay. To evaluate the reproducibility of the test, we selected four sperm samples scale of 0 to +++ according to the distribution of fluorescence label. Results: The results were highly correlated with the corrected total cell fluorescence (CTCF) (Rp = 0.9972, P < 0.05). We suggest that the relationship between infertility and fertilin ? may be due to the distribution of this protein on the sperm surface. Male partners of couples with unexplained infertility showed a low distribution of fertilin ? by a decrease of the fluorescence signal in the IF labeling (scale of +++ by 7.4 ± 10.32%, P < 0.0001, ±SD). Discussion: Abnormal fertilin ? function may be a potential mechanism that could lead to fertilization failure. © 2022 Asociación Española de Andrología, Medicina Sexual y Reproductiva
Açıklama
Anahtar Kelimeler
Andrology; Fertility; Oligozoospermia; Sperm; Surface proteins; Unexplained infertility
Kaynak
Revista Internacional de Andrologia
WoS Q Değeri
Scopus Q Değeri
Q3
Cilt
20
Sayı
4
